Everything, everywhere, all at once.
SimulScreen gives us the ability to run multiple assays for all of the antibodies, all at the same time. For instance:
Have a related molecule that you don’t want your antibody to bind to? Want to make sure it’s specific to your preferred form of target? We capture Abs on 1 slide, stain first with the non-preferred form and then the preferred form. A subtraction of images on the 2 stains reveals new hits specific to your preferred molecule.
Have a related molecule that you do want your antibody to bind to? We capture Abs on 2 slides and stain with each form. We then align the images from the 2 slides to find the cross-reactive Abs.
You have a receptor or ligand that you want to block? We capture antibodies on 2 slides - on 1 we confirm binding to the target, on the 2nd we stain all binders with the target and subsequently try to stain with receptor - if it binds, the Ab doesn’t block the epitope. If it doesn’t bind, it’s a potential blocker.
Have a benchmark antibody that is functional or binds to an epitope of interest? We capture antibodies on 2 slides; on the first we confirm binding to the target, on the second we stain all binders with the target and then try to stain with benchmark Ab. If it binds, the captured Ab doesn’t block its epitope. If it doesn’t bind, it’s a potential competing Ab.