---
title: Antigen Enrichment
description: "Single Cell Technology case studies: Using Microfluidics-based cell sorter to enrich antigen-specific antibodies for Antibody Discovery"
---

[Resources ](https://singlecelltechnology.com/resources)

# [Antigen Enrichment](https://singlecelltechnology.com/resources/antigen-enrichment-for-antibody-discovery)

 Written by [Single Cell Technology](https://singlecelltechnology.com/resources/author/single-cell-technology) | 21 January 2025

### The Challenge 

- Increasing hit rates in antibody discovery campaigns 

### The Single Cell Solution

- Study markers in mouse cells for antibody-secreting cells
- Develop a workflow for enriching antigen-specific antibodies 
- Test workflow on therapeutic targets to build use cases 

### Highlights

- Increase hit rate and diversity of hits
- Include in AbTheneum discovery workflow, integrating dynamic screening

*[View the Poster >>](https://singlecelltechnology.com/hubfs/2024AET%20Single%20Cell%20-%20FACS%20%26%20Furious.pdf)*

## Solution: Driving More Hits

### Antibody Discovery Bottleneck

Certain targets in antibody discovery present challenges because they produce few hits. This can be due several reasons: immunogenicity of the target, immunomodulatory effects of the target during immunization, the immunization conditions used, and/or the discovery methods used. Furthermore, some antibody programs demand highly specialized activity for their antibody drugs, making it diffult to discover antibody leads with the desired function. We believe – and our Clients agree – that exploring a large diverse set of hits is the best start to an antibody campaign. 

How can we drive more hits? 

We apply our FACS antigen enrichment workflow to discover more hits. This enrichment method consistently produces increased hits in every campaign we've thrown at it.

Learn more by watching our presentation, delivered at AET US 2024

[View full post](https://singlecelltechnology.com/resources/antigen-enrichment-for-antibody-discovery)

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